Part:BBa_K2974310:Experience

The results for the toehold were confirmed through a dual plasmid transformation in BL21 E. coli. The dual plasmid transformation presented greater fluorescence measured in lux values in comparison to the single switch transformation, confirming the function of the toehold. The dual plasmid transformation would not succeed without the correct C. elegans toehold intermediate, so the function of this part (BBa_K2974310) has been validated through experimentation and sequencing (see BBa_K2974316 for sequencing confirmation).

Figure 1: This graph compares the mean lux values of the C. elegans toehold switch, trigger, and the dual plasmid. The toehold switch and trigger have very similar lux values to LB and plain cells, whereas the dual plasmid has a higher lux value, indicating fluorescence.

Applications of BBa_K2974310

This sequence is to be used as a proof of concept, and can be used to develop biosensor control devices.

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